OrangerV1, Main, Exploration, bibRecord, 003596

Characterisation of protoplasts from hypocotyls of Helianthus annuus in relation to their tissue origin

Identifieur interne : 003596 ( Main/Exploration ); précédent : 003595; suivant : 003597

Characterisation of protoplasts from hypocotyls of Helianthus annuus in relation to their tissue origin

Auteurs : M. Petitprez [France] ; C. Brière [France] ; C. Borin [France] ; J. Kallerhoff [France] ; A. Souvré [France] ; G. Alibert [France]

Source :

Plant Cell, Tissue and Organ Culture [ 0167-6857 ] ; 1995-04-01.

RBID : ISTEX:FEB8B3804875C42F834844B0ED925541008CBCA8

Abstract

Abstract: Cells and protoplasts isolated from three different tissues of sunflower hypocotyls and cultured either in liquid or agarose medium were compared in terms of their volume, DNA content, division potential and embryoid formation. Epidermal and external cortical cells differ from other tissue cells by their small size, their weak response to plasmolysis and their low DNA content (around 1C). They contribute only very weakly to the dividing protoplast population. In contrast, protoplasts from cortical and medullar cells both have similar division potential, reaching 50%. The nuclear DNA content of these two cell types, as well as their corresponding protoplasts, has a 2C value, taking root tip cells in G0 phase as standard. The culture conditions induce the same specific response in protoplasts isolated from both tissues: exclusively loose colony formation in liquid medium, and mainly production of embryoids in agarose medium.

Url:

https://api.istex.fr/document/FEB8B3804875C42F834844B0ED925541008CBCA8/fulltext/pdf

DOI: 10.1007/BF00124084

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 000959
to stream Istex, to step Curation: 000959
to stream Istex, to step Checkpoint: 000F03
to stream Main, to step Merge: 003863
to stream Main, to step Curation: 003596

Le document en format XML

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<author><name sortKey="Borin, C" sort="Borin, C" uniqKey="Borin C" first="C." last="Borin">C. Borin</name>
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<author><name sortKey="Kallerhoff, J" sort="Kallerhoff, J" uniqKey="Kallerhoff J" first="J." last="Kallerhoff">J. Kallerhoff</name>
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<front><div type="abstract" xml:lang="en">Abstract: Cells and protoplasts isolated from three different tissues of sunflower hypocotyls and cultured either in liquid or agarose medium were compared in terms of their volume, DNA content, division potential and embryoid formation. Epidermal and external cortical cells differ from other tissue cells by their small size, their weak response to plasmolysis and their low DNA content (around 1C). They contribute only very weakly to the dividing protoplast population. In contrast, protoplasts from cortical and medullar cells both have similar division potential, reaching 50%. The nuclear DNA content of these two cell types, as well as their corresponding protoplasts, has a 2C value, taking root tip cells in G0 phase as standard. The culture conditions induce the same specific response in protoplasts isolated from both tissues: exclusively loose colony formation in liquid medium, and mainly production of embryoids in agarose medium.</div>
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Characterisation of protoplasts from hypocotyls of Helianthus annuus in relation to their tissue origin

Characterisation of protoplasts from hypocotyls of Helianthus annuus in relation to their tissue origin

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri